chromogen  (Vector Laboratories)

Journal: bioRxiv

Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

doi: 10.64898/2026.04.16.718819

Figure Lengend Snippet: Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, and Ki-67 expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.

Article Snippet: Sections were blocked with 3% H2O2, respectively incubated with CK7, TTF-1, Ki-67 and mesothelin primary antibodies at 4°C overnight, followed by secondary antibodies and DAB substate (C09-12, OriGene).

Techniques: Derivative Assay, Immunohistochemical staining, Staining, Immunohistochemistry, Expressing, Immunofluorescence, Mutagenesis

Journal: bioRxiv

Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

doi: 10.64898/2026.04.16.718819

Figure Lengend Snippet: Histological and Molecular Concordance Analysis of Tumor Tissue and Organoid. (A) Histological and immunohistochemical concordance of tumor tissue and organoids from Patient 1 and Patient 2. H&E staining shows the overall histological structure, while IHC for CK7, TTF-1, and Ki-67 demonstrates the expression of these markers in both tissue and organoid samples. Scale bars represent 100 μm. (B) Fluorescence microscopy images of Patient 6 organoids. DAPI staining (blue) highlights the nuclei, F-actin staining (red) shows the cytoskeleton, and immunofluorescence for NapsinA, CK7, TTF-1, and Ki-67 (green) indicates the expression and localization of these proteins. Scale bars represent 100 μm. (C) Heatmap showing the correlation between gene expression profiles of tumor (T) and organoid (O) samples from five patients. The color scale ranges from blue (low correlation) to red (high correlation), indicating the similarity in gene expression between corresponding tumor and organoid samples. (D) Mutation profile comparison between tumor (T) and organoid (O) samples. (E) DNA copy number variation (CNV) analysis of Patient 1 and Patient 2 between tumor (T) and organoid (O) samples.

Article Snippet: Sections were blocked with 3% H2O2, respectively incubated with CK7, TTF-1, Ki-67 and mesothelin primary antibodies at 4°C overnight, followed by secondary antibodies and DAB substate (C09-12, OriGene).

Techniques: Immunohistochemical staining, Staining, Expressing, Fluorescence, Microscopy, Immunofluorescence, Gene Expression, Mutagenesis, Comparison